Long-distance movement factor: a transport function of the potyvirus helper component proteinase.
نویسندگان
چکیده
منابع مشابه
Long-distance movement factor: a transport function of the potyvirus helper component proteinase.
Transport of viruses from cell to cell in plants typically involves one or more viral proteins that supply dedicated movement functions. Transport from leaf to leaf through phloem, or long-distance transport, is a poorly understood process with requirements differing from those of cell-to-cell movement. Through genetic analysis of tobacco etch virus (TEV; potyvirus group), a novel long-distance...
متن کاملCapsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins.
The role of bean common mosaic necrosis potyvirus (BCMNV) and lettuce mosaic potyvirus (LMV) proteins was investigated in terms of their capacity to function as viral movement proteins (MPs). Using Escherichia coli-expressed proteins and microinjection techniques, direct evidence was obtained that both the potyviral capsid protein (CP) and helper component- proteinase (HC-Pro) function in this ...
متن کاملEffect of mutations within the cys-rich region of potyvirus helper component-proteinase on self-interaction.
The first approximately 60 amino acids of the N-terminal part of the potyvirus helper component-proteinase (HC-Pro) include highly conserved residues comprising a Cys-rich region. In the present study, the domain in Potato virus Y sufficient for self-interaction was mapped using the yeast two-hybrid system to the 83 N-terminal amino acids of HC-Pro. Mutations in the conserved His and two Cys re...
متن کاملStructure of the autocatalytic cysteine protease domain of potyvirus helper-component proteinase.
The helper-component proteinase (HC-Pro) of potyvirus is involved in polyprotein processing, aphid transmission, and suppression of antiviral RNA silencing. There is no high resolution structure reported for any part of HC-Pro, hindering mechanistic understanding of its multiple functions. We have determined the crystal structure of the cysteine protease domain of HC-Pro from turnip mosaic viru...
متن کاملAutocatalytic processing of the potyvirus helper component proteinase in Escherichia coli and in vitro.
The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known cleavage event catalyzed by HC-Pro occurs at the HC-Pro carboxyl terminus. The pro...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1995
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.7.5.549